| Program and Presentation
downloads qPCR Satellite Symposium 10 - 11th March 2005 |
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Thursday 10th March 2005 8:00 – 9:00 Arrival & Registration 9:00 – 9:30 Welcome by the Organizers & Opening of the Satellite-Symposium, Michael W. Pfaffl |
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9:30 – 12:45 Session 1: Pre-analytical steps Moderator: M. W. Pfaffl 9:30 – 10:15 Neven Zoric, Coordinator of the TATAA Biocenter, Göteborg, Sweden What to do and not to do in reverse transcription ! 10:15 – 10:45 Thomas Kaiser, Corbett Research R&D, Sydney, Australia DNA Preparation on the X-tractor-Gene and pre-PCR setup on the CAS 1200. 10:45 – 11:15 Coffee break 11:15 – 11:45 Debra Ann Nickson, European Senior Product Manager, Genomics, Invitrogen Europe, Paisley, Scotland Improving cDNA yields using SuperScript III. 11:45 – 12:15 Marc Valer, Agilent Technologies, Waldbronn, Germany Chip based analysis of nucleic acids combined with RNA Integrity Number (RIN) algorithm enables fast, independent measurement of RNA quality. 12:15 – 12:45 Marc Valer, Agilent Technologies, Waldbronn, Germany Practical demonstration of the Bioanalyzer 2100. 12:45 – 13:45 Lunch and Poster Session: Applied Biosystems, Artus Biotech, Exiqon, Premier Biosoft and Qiagen |
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13:45 – 15:30 Session 2: Single cell PCR Moderator: M. Kubista 13:45 – 14:30 Stephen A. Bustin, Institute of Cell & Mol. Science, Queen Mary's School of Medicine & Dentistry, University of London Laser capture micro dissection for biologically meaningful quantification of mRNA. 14:30 – 15:00 Anders Stahlberg, Department of Chemistry and Biosciences, Chalmers University of Technology, Sweden Gene Expression Profiling - From Tissue to Single cell. 15:00 – 15:30 Tanja Nolan, Sigma-Aldrich Chemie Gmbh, Munich, Germany Accurate gene expression profiling from limiting material - facing the issues of normalization, efficiency and mRNA to protein correlation. 15:30 – 16:15 Coffee break |
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16:15 – 18:15 Session 3: Probes Technologies Moderator: S. Bustin 16:15 – 16:45 Olfert Landt, TIB MOLBIOL Syntheselabor GmbH, Berlin, Germany Clamped-Probe-Assay: Detection and quantification of minor/minimal variants. 16:45 – 17:15 Ralf Mauritz, Roche Applied Science, Diagnostics, Penzberg, Germany ProbeLibrary: One Transcriptome - One Kit. 17:15 – 17:45 Andres Jarrin, Eppendorf, Germany Improved Real Time PCR Application using Novel Eppendorf Amplification Technologies. 17:45 – 18:15 Uta Möllers, Application Specialist, Applied Biosystems TaqMan Assays - ....not only Primers and Probes. 20:00 –23:30 Banquet in the Auerbachs Keller in the city center of Leipzig http://www.auerbachs-keller-leipzig.de |
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Friday 11th March 2005 9:00 – 12:15 Session 4: Quantification strategies & Optimization Moderator: S. Bustin 9:00 – 9:45 Michael W. Pfaffl, Center of Life Science, Weihenstephan, Technical University of Munich, Germany Quantification strategies in real-time RT-PCR. 9:45 –10:15 Mary Span, EUROGENTEC, Liege science park, Seraing, Belgium Pitfalls in qPCR. 10:15 – 10:45 Thomas Kaiser, Corbett Research R&D, Sydney, Australia qPCR normalization and optimization in the Rotor-Gene 3000. 10:45 – 11:15 Coffee break 11:15 – 11:45 Jim Huggett, Centre for Infectious Diseases & International Health, University College London, UK Real time RT-PCR normalisation: methods and considerations. 11:45 – 12:15 Reinhold Müller, Stratagene R&D, La Jolla, USA Considerations for standardizing qPCR assays. 12:15 – 13:15 Lunch and Poster Session: Applied Biosystems, Artus Biotech, Exiqon, Premier Biosoft and Qiagen |
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13:15 – 14:45 Session 5: Applications Moderator: N. Zoric 13:15 – 13:45 Tomasz Czechowski, Molecular Plant Nutrition Group, MPI for Molecular Plantphysiology Golm, Germany Real time RT-PCR profiling of over 1,400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel tissue-specific genes. 13:45– 14:15 Hilary Srere, Bio-Rad Laboratories Europe, Munich, Germany The Ups and Downs of Gene Expression: Characterization of New Tools Used for Gene Expression and Knockdown. 14:15 – 14:45 Linda Vigilant, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany Applications of qPCR to genetic analyses of wild primates. 14:45 – 15:15 Coffee break |
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15:15 – 17:15 Session 6: Post PCR & BioInformatics Moderator: M. W. Pfaffl 15:15 – 16:00 Mikael Kubista, Department of Chemistry and Biosciences, MultiD Analyses AB, Göteborg, Sweden Gene expression analysis. 16:00 – 16:30 Jo Vandesompele, Center for Medical Genetics Ghent, Ghent University Hospital Gene expression profiling: Accurate normalization and automated data-analysis. 16:30 – 17:00 Yevhen Vainshtein, EMBL Heidelberg, Meyerhofstrasse, Heidelberg, Germany REST-384: an improved way of simple and precise qRT-PCR data evaluation. 17:00 – 17:15 Closing of the Symposium, Michael W. Pfaffl |
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further
information on the qPCR Symposium via leipzig05@bioscience-events.com
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